| 出品公司: | Promega |
|---|---|
| 载体名称: | pNL1.1[Nluc] |
| 质粒类型: | 荧光素酶载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | CMV |
| 载体大小: | 3110 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
![pNL1.1[Nluc]载体图谱](/uploads/载体图谱/pNL1.1[Nluc]载体图谱.png)
载体简介
载体序列
LOCUS pNL1.1[Nluc] 3110 bp ds-DNA circular SYN 26-JUL-2013
DEFINITION Promoterless vector for measuring the activity of promoter and
enhancer sequences with a NanoLuc(R) luciferase assay.
ACCESSION JQ437370
VERSION .
KEYWORDS pNL1.1[Nluc]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3110)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Saturday, June 6, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3110
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
misc_feature 1..70
/note="MCS"
/note="multiple cloning site"
CDS 100..615
/codon_start=1
/product="NanoLuc(R) luciferase"
/note="Nluc"
/note="human codon-optimized"
/translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
RLCERILA"
polyA_signal 664..785
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1204..1792)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1992..2852)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 2957..3005
/note="synthetic polyadenylation signal"
misc_feature 3019..3110
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggtcttcac actcgaagat
121 ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct tgaacaggga
181 ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc
241 ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta tgaaggtctg
301 agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc tgtggatgat
361 catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt tacgccgaac
421 atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg caaaaagatc
481 actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct gatcaacccc
541 gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg gctgtgcgaa
601 cgcattctgg cgtaaggccg cgactctaga gtcggggcgg ccggccgctt cgagcagaca
661 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
721 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
781 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
841 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg
901 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
961 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
1021 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
1081 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
1141 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
1201 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
1261 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
1321 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
1381 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
1441 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
1501 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
1561 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
1621 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
1681 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
1741 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
1801 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
1861 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
1921 taaatcaatc taaagtatat atgagtaaac ttggtctgac agcggccgca aatgctaaac
1981 cactgcagtg gttaccagtg cttgatcagt gaggcaccga tctcagcgat ctgcctattt
2041 cgttcgtcca tagtggcctg actccccgtc gtgtagatca ctacgattcg tgagggctta
2101 ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc gttcaccggc ccccgatttg
2161 tcagcaatga accagccagc agggagggcc gagcgaagaa gtggtcctgc tactttgtcc
2221 gcctccatcc agtctatgag ctgctgtcgt gatgctagag taagaagttc gccagtgagt
2281 agtttccgaa gagttgtggc cattgctact ggcatcgtgg tatcacgctc gtcgttcggt
2341 atggcttcgt tcaactctgg ttcccagcgg tcaagccggg tcacatgatc acccatatta
2401 tgaagaaatg cagtcagctc cttagggcct ccgatcgttg tcagaagtaa gttggccgcg
2461 gtgttgtcgc tcatggtaat ggcagcacta cacaattctc ttaccgtcat gccatccgta
2521 agatgctttt ccgtgaccgg cgagtactca accaagtcgt tttgtgagta gtgtatacgg
2581 cgaccaagct gctcttgccc ggcgtctata cgggacaaca ccgcgccaca tagcagtact
2641 ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga aagactcaag gatcttgccg
2701 ctattgagat ccagttcgat atagcccact cttgcaccca gttgatcttc agcatctttt
2761 actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga
2821 atgagtgcga cacgaaaatg ttggatgctc atactcgtcc tttttcaata ttattgaagc
2881 atttatcagg gttactagta cgtctctcaa ggataagtaa gtaatattaa ggtacgggag
2941 gtattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
3001 gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact
3061 agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
//
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